HemoTypeSC point-of-care testing shows high sensitivity with alkaline cellulose acetate hemoglobin electrophoresis for screening hemoglobin SS and SC genotypes

Comparative analysis of cellulose acetate hemoglobin electrophoresis and high performance liquid chromatography for quantitative determination of hemoglobin A2

BACKGROUND The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels. METHODS The test population comprised 160 individuals divided into four groups: normal individuals, β-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, a...

Correlation between Standard Clinical Chemistry and Point of Care Testing Analyzers on Hemoglobin A1C Determination

Accuracy of noninvasive hemoglobin and invasive point-of-care hemoglobin testing compared with a laboratory analyzer

INTRODUCTION Hemoglobin concentration is assessed to detect anemia and its associated morbidities. Hemoglobin is usually determined from venous or capillary blood samples run on a laboratory analyzer. However, this method requires a needle stick and results can be delayed. It also exposes caregivers to risks associated with needle sticks and blood exposure. Noninvasive hemoglobin determination ...

Comparison of electrophoresis on citrate agar, cellulose acetate, or starch for hemoglobin identification.

We compare and discuss three electrophoretic methods for identifying hemoglobins S, A, C, F, and D or G. Electrophoresis on citrate agar gel was more sensitive than electrophoresis on cellulose acetate for detecting hemoglobins S and F, a fundamental consideration in designing cord-blood screening programs for detecting hemoglobin S carriers. Electrophoresis on starch gel is evidently an accept...

BRIEF REPORT Rapid Differentiation of Polypeptide Chains of Hemoglobin by Cellulose Acetate Electrophoresis of Hemolylsates

I DENTIFICATION of the abnormal polypeptide chain of a hemoglobin ( Hb ) variant is often useful in connection with structural analyses and Chernoff and Pettit’ have recently improved on existing methods for this purpose by using starch gel electrophoresis of globin in urea barbital buffer. Izzo2 used this same buffer for electrophoresis of globin on cellulose acetate. Ohba et al.3 have propose...